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avidin dcs rhodamine  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin dcs rhodamine
    Avidin Dcs Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhodamine+avidin+dcs/Rhodamine+Avidin+DCS%2C+Cell+Sorting+Grade/pm38062355-117-15-20
    Average 93 stars, based on 52 article reviews
    avidin dcs rhodamine - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Functional correction of dystrophin actin binding domain mutations by genome editing
    Article Snippet: Following overnight incubation at 4°C, unbound primary antibodies were removed with PBS washes, and coverslips were probed for 1 hour with secondary antibodies, biotinylated horse anti–mouse IgG (1:200) (catalog BA-2000, Vector Laboratories) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (catalog 711-095-152, Jackson ImmunoResearch) diluted in 0.2% BSA/PBS. .. Unbound secondary antibodies were removed with PBS washes, and final dystrophin labeling was done with 10-minute incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in PBS. .. Unbound rhodamine was removed with PBS washes, nuclei were counterstained with 2 μg/ml Hoechst 33342 (Molecular Probes), and coverslips were inverted onto slides overlaid with Vectashield fluorescent mounting media (Vector Laboratories).

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing.
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluoresceinconjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Incubation:

    Article Title: Functional correction of dystrophin actin binding domain mutations by genome editing
    Article Snippet: Following overnight incubation at 4°C, unbound primary antibodies were removed with PBS washes, and coverslips were probed for 1 hour with secondary antibodies, biotinylated horse anti–mouse IgG (1:200) (catalog BA-2000, Vector Laboratories) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (catalog 711-095-152, Jackson ImmunoResearch) diluted in 0.2% BSA/PBS. .. Unbound secondary antibodies were removed with PBS washes, and final dystrophin labeling was done with 10-minute incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in PBS. .. Unbound rhodamine was removed with PBS washes, nuclei were counterstained with 2 μg/ml Hoechst 33342 (Molecular Probes), and coverslips were inverted onto slides overlaid with Vectashield fluorescent mounting media (Vector Laboratories).

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing.
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluoresceinconjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Peripheral tetrahydrobiopterin is involved in the pathogenesis of mechanical hypersensitivity in a rodent postsurgical pain model.
    Article Snippet: Because treatment for postsurgical pain (PSP) remains a major unmet medical need, the emergence of safe and innovative nonopioid drugs has been strongly coveted.. Tetrahydrobiopterin (BH4) is an interesting molecule for gaining a better understanding the pathological mechanism of neuropathic pain.. However, whether BH4 and its pathway are involved in the pathogenesis of PSP remains unclear.

    Article Title: TrkB/BDNF Signaling Regulates Photoreceptor Progenitor Cell Fate Decisions
    Article Snippet: Sections were double-labeled using polyclonal antisera specific for C-terminal amino acids of trunc TrkB but not in full length TrkB (1:50, sc-119, Santa Cruz Biotechnology) and a monoclonal antibody against β-galactosidase (1:500; Sigma, St Louis, MO). .. After washing, sections were incubated in FITC-conjugated goat anti-rabbit IgG (1:400; Jackson Immunoresearch Laboratories, West Grove, PA) and biotinylated horse anti-mouse IgG (1:400; Vector Laboratories) followed by incubation in rhodamine avidin DCS (1:500, Vector Laboratories). .. Sections were mounted in Vectashield (Vector Laboratories) and examined using a Nikon epifluorescence microscope.

    Avidin-Biotin Assay:

    Article Title: Functional correction of dystrophin actin binding domain mutations by genome editing
    Article Snippet: Following overnight incubation at 4°C, unbound primary antibodies were removed with PBS washes, and coverslips were probed for 1 hour with secondary antibodies, biotinylated horse anti–mouse IgG (1:200) (catalog BA-2000, Vector Laboratories) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (catalog 711-095-152, Jackson ImmunoResearch) diluted in 0.2% BSA/PBS. .. Unbound secondary antibodies were removed with PBS washes, and final dystrophin labeling was done with 10-minute incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in PBS. .. Unbound rhodamine was removed with PBS washes, nuclei were counterstained with 2 μg/ml Hoechst 33342 (Molecular Probes), and coverslips were inverted onto slides overlaid with Vectashield fluorescent mounting media (Vector Laboratories).

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing.
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluoresceinconjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Transient Global Cerebral Ischemia Induces RNF213, a Moyamoya Disease Susceptibility Gene, in Vulnerable Neurons of the Rat Hippocampus CA1 Subregion and Ischemic Cortex.
    Article Snippet: The RING finger protein 213 (RNF213) is an important susceptibility gene for moyamoya disease (MMD) and is also implicated in other types of intracranial major artery stenosis/occlusion (ICAS); however, the role of RNF213 in the development of ICAS including MMD is unclear.. The constitutive expression of the RNF213 gene is relatively weak in brain tissue, while information regarding the expression patterns of the RNF213 gene under cerebral ischemia, which is one of characteristic pathologies associated with ICAS, is currently limited.. Our objective was to address this critical issue, and we investigated Rnf213 mRNA expression in rat brains after 5 minutes of transient global cerebral ischemia (tGCI) by occluding the common carotid arteries coupled with severe hypotension.

    Article Title: Peripheral tetrahydrobiopterin is involved in the pathogenesis of mechanical hypersensitivity in a rodent postsurgical pain model.
    Article Snippet: Because treatment for postsurgical pain (PSP) remains a major unmet medical need, the emergence of safe and innovative nonopioid drugs has been strongly coveted.. Tetrahydrobiopterin (BH4) is an interesting molecule for gaining a better understanding the pathological mechanism of neuropathic pain.. However, whether BH4 and its pathway are involved in the pathogenesis of PSP remains unclear.

    Article Title: TrkB/BDNF Signaling Regulates Photoreceptor Progenitor Cell Fate Decisions
    Article Snippet: Sections were double-labeled using polyclonal antisera specific for C-terminal amino acids of trunc TrkB but not in full length TrkB (1:50, sc-119, Santa Cruz Biotechnology) and a monoclonal antibody against β-galactosidase (1:500; Sigma, St Louis, MO). .. After washing, sections were incubated in FITC-conjugated goat anti-rabbit IgG (1:400; Jackson Immunoresearch Laboratories, West Grove, PA) and biotinylated horse anti-mouse IgG (1:400; Vector Laboratories) followed by incubation in rhodamine avidin DCS (1:500, Vector Laboratories). .. Sections were mounted in Vectashield (Vector Laboratories) and examined using a Nikon epifluorescence microscope.

    Saline:

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluorescein-conjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing.
    Article Snippet: Then, coverslips were probed for 1 hour with biotinylated horse anti-mouse immunoglobulin G (IgG) (1:200) (Vector Laboratories, BA-2000) and fluoresceinconjugated donkey anti-rabbit IgG (1:50) (Jackson ImmunoResearch, 711-095-152) diluted in 0.2% BSA/phosphate-buffered saline. .. Unbound secondary antibodies were removed with phosphate-buffered saline washes, and final dystrophin labeling was done with a 10-min incubation of rhodamine avidin DCS (1:60) (Vector Laboratories) diluted in phosphate-buffered saline. ..

    Article Title: Peripheral tetrahydrobiopterin is involved in the pathogenesis of mechanical hypersensitivity in a rodent postsurgical pain model.
    Article Snippet: Because treatment for postsurgical pain (PSP) remains a major unmet medical need, the emergence of safe and innovative nonopioid drugs has been strongly coveted.. Tetrahydrobiopterin (BH4) is an interesting molecule for gaining a better understanding the pathological mechanism of neuropathic pain.. However, whether BH4 and its pathway are involved in the pathogenesis of PSP remains unclear.



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